circrnas chip arraystar human circrnas chip  (Arraystar inc)

Journal: Molecular Therapy. Nucleic Acids

Article Title: The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms

doi: 10.1016/j.omtn.2023.08.017

Figure Lengend Snippet: Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

Article Snippet: The resulting labeled cDNA was then purified and 1 μg was fragmented, heated, and subsequently hybridized with an 8 × 15k commercially available array chip displaying 13,617 human circRNAs (Arraystar, no. AS-S-CR-H-V2.0) for 17 h at 65°C in an Agilent Hybridization Oven.

Techniques: Control, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative Proteomics

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: circRNA_141539 was up-regulated in ESCC tissue. ( A ) Hierarchical clustering analysis showed the top 10 most up-regulated and down-regulated circRNAs. The expression levels are presented in different colors indicating expression levels. ( B , C ) qRT-PCR assay showed that circRNA_001937 was significantly up-regulated in ESCC tissues (n=50), * P < 0.05, ** P < 0.01 versus corresponding adjacent tissues. circRNA_141539 expression levels were normalized to GAPDH. N: adjacent normal tissues, C: ESCC tissues.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques: Expressing, Quantitative RT-PCR

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: circRNA_141539 was associated with diagnosis and prognosis in ESCC. ( A ) The median expression of circRNA_141539. ( B ) ROC curve showed the diagnostic efficiency of circRNA_141539. ( C ) Kaplan- Meier progression-free survival curves showed the association between high and low circRNA_141539 expression.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques: Biomarker Discovery, Expressing, Diagnostic Assay

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: Multivariate analyses for overall survival by Cox regression test.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques:

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: circRNA_141539 was associated with clinicopathological characteristics in ESCC. ( A ) The association of circRNA_141539 with TNM stage, ( B ) with T stage, ( C ) with N stage, and ( D ) with histological grade.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques:

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: circRNA_141539 can promote cell proliferation and invasion (n=3). ( A ) MTT assay; ( B ) Transwell chamber invasion assay. * P < 0.05, ** P < 0.01 versus corresponding NC group.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques: MTT Assay, Invasion Assay

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: circRNA_141539 acts as a miRNA sponge for miR-4469. ( A ) Schematic diagram showed that miR-4469 specifically binds to 3′-UTR of circRNA_141539. ( B ) Dual-luciferase assay results. ( C ) qRT-PCR showed miR-4469 was were significantly down-regulated in ESCC tissues (n=50), * P < 0.05, ** P < 0.01 versus corresponding adjacent tissues. ( D ) Pearson's correlation showed that circRNA_141539 was negatively correlated to miR-4469 expression. ( E , F ) qRT-PCR showed miR-4469 were significantly decreased in circRNA_141539 OE group, and dramatically increased in sh-circRNA_141539 group. * P < 0.05, ** P < 0.01 versus corresponding NC group.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques: Luciferase, Quantitative RT-PCR, Expressing

Journal: Aging (Albany NY)

Article Title: circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

doi: 10.18632/aging.103071

Figure Lengend Snippet: Rescue experiment, miR-4469 reversed the effects of circRNA_141539 on CDK3 expression and cell proliferation. ( A ) qRT-PCR assay showed the effects of circRNA_141539/miR-4469 axis on CDK3 mRNA expression. * P < 0.05, ** P < 0.01 versus corresponding NC group. ( B ) MTT assay showed the effects of circRNA_141539/miR-4469 axis on cell proliferation. * P < 0.05, ** P < 0.01 versus corresponding NC group.

Article Snippet: CircRNAs were subsequently enriched and linear RNAs were removed using the Rnase R. Arraystar Human circRNA chip (8x15 K; Arraystar, Inc.).

Techniques: Expressing, Quantitative RT-PCR, MTT Assay